SMARCA4/BRG1 Inhibitor Screening Assay Kit

• Cat.No.: Kit-1867

Specification

• Size: 384 reactions
• Description: The BRG1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRG1 binding to its substrate. The key to the BRG1 Inhibitor Screening Assay Kit is the specific binding of the BRG1 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRG1 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
• Applications: Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
• Storage: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Kit Components: SMARCA4/BRG1 (1480-1603): 20 µg; -80°CBromodomain Ligand 2 (BRG1): 400 µl; -80°C Non-acetylated Ligand 2 (BRG1): 200 µl; -80°C 3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C
• Tag: Non