Sirtuin Activity Fluorometric Assay Kit

• Cat.No.: KITZ-049
• Product Overview: Sirtuin Activity Fluorometric Assay Kit is suitable for measuring activity/inhibition of total
  SIRT enzyme using nuclear extracts or purified SIRT isoforms (SIRTs1-7) from a broad
  range of species such as mammals, plants, fungi, and bacteria, in a variety of forms
  including, but not limited to cultured cells, fresh and frozen tissues. For SIRT1, SIRT6,
  and SIRT7, nuclear extracts are required and can be prepared by using your own
  successful method. For SIRT2, SIRT4, and SIRT5, cytoplasmic extracts are required.
  For SIRT3, mitocondria fractions should be used. Purified enzymes can be active SIRTs
  from recombinant proteins or isolated from cell/tissues.

Specification

• Size: 48 Assays or 96 Assays
• Storage: The kit is shipped in two parts: the first part at ambient room temperature and the second part on frozen ice packs at 4°C.
  
• Shipping: Ice pack
• Kit Components: WB (10X Wash Buffer): 14 ml for 48 Assays, 28 ml for 96 Assays, 4°C
  HO1 (SIRT Assay Buffer): 4 ml for 48 Assays, 8 ml for 96 Assays, RT
  HO2 (SIRT Substrate, 50X): 60 μl for 48 Assays, 120 μl for 96 Assays, -20°C
  HO3 (SIRT Assay Standard, 50μg/ml): 10 μl for 48 Assays, 20 μl for 96 Assays, -20°C
  HO4 (Capture Antibody, 1000X): 5 μl for 48 Assays, 10 μl for 96 Assays, 4°C
  HO5 (Detection Antibody, 2000X): 6 μl for 48 Assays, 12 μl for 96 Assays, -20°C
  NAD (SIRT Co-factor, 50X): 50 μl for 48 Assays, 100 μl for 96 Assays, -20°C
  TSA (HDAC inhibitor, 50μM): 50 μl for 48 Assays, 100 μl for 96 Assays, -20°C
  NAM (SIRT Inhibitor, 50 mM): 40 μl for 48 Assays, 80 μl for 96 Assays, -20°C
  FD (Fluoro Developer): 10 μl for 48 Assays, 20 μl for 96 Assays, -20°C
  FE (Fluoro Enhancer): 10 μl for 48 Assays, 20 μl for 96 Assays, 4°C
  DB (Dilution Buffer): 4 ml for 48 Assays, 8 ml for 96 Assays, RT
  8-Well Assay Strips (With Frame): 6 for 48 Assays, 12 for 96 Assays, 4°C
  Adhesive Covering Film: 1 for 48 Assays, 1 for 96 Assays, RT
• Materials Required but Not Supplied: Adjustable pipette or multiple-channel pipette
  Multiple-channel pipette reservoirs
  Aerosol resistant pipette tips
  Fluorescence microplate reader capable of reading fluorescence at 530ex/590em nm
  1.5ml microcentrifuge tubes
  Incubator for 37°C incubation
  Distilled water
  Nuclear extract or purified enzymes
  Parafilm M or aluminum foil