SARS-CoV-2 (COVID-19) Spike protein RBD-coupled magnetic beads

• Cat.No.: RBD-002M
• Product Overview: The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.

Specification

• Species: SARS-CoV-2
• Beads Size: 2 mg
• Particle size: 2 μm
• Coupled amount of protein: 80 μg
• Capacity: > 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose
• Reconstitution: 2 mL ultrapure water (1mg beads/mL)
• Application: This product is intended for immunocapture, biopanning and flow cytometry.
• Storage: Upon receipt, please store the Beads at -20ºC for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately. Do not to freeze thaw the Beads after reconstitution.
• Assay Principles: The Antigen pre-Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike protein RBD coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as immunocapture, biopanning and flow cytometry.
• Application Method: a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
  b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.