RNA pull-down Kit

• Cat.No.: KITMI-RP07
• Product Overview: RNA pull-down is a technique to study the binding of RNA to proteins or the binding to RNA in cells. The RNA is labeled with biotin labeling and then co-incubated with the cell lysate to form an RNA-RNA or RNA-protein complex. Incubate with magnetic beads that coupled with streptavidin, the RNA-RNA or RNA-protein complex will be enriched and eluted. Fluorescence quantitative PCR (RNA pull down- QPCR) or high-throughput sequencing (RNA pull down-seq) is used to determine whether the target RNA interacts with some RNA molecules. Western blot (pull down-WB) assay and mass spectrometry (pull down-MS) is used to detect whether the target RNA interacts with the proteins.

Specification

• Size: 6 tests
• Storage: Protease inhibitor needs to be stored at -20°C and the rest stored at 4°C
• Applications: Study the protein and RNA interactions
• Notes: 6 tests are 6 times of single-group (1 experimental group or 1 control group) immunoprecipitation experiment. The following steps include 1 group of experimental group and 1 group of control group, which needs to consume 2 test reagents.
• Shipping: On Ice
• Kit Components: RNA binding buffer: 6mL
  Wash Buffer I: 20mL
  Protein-RNA binding buffer (10X): 600uL
  IP lysis buffer : 9mL
  Protease inhibitor (100 X): 35uL
  Glycerol (50%): 800uL
  Wash Buffer II: 10mL
  Solution I: 600uL
  Elution Buffer: 800uL
  DEPC water: 400uL
  Nucleic-Acid Compatible Streptavidin Magnetic Beads: 270uL
• Materials Required but Not Supplied: 75% ethanol, Trizol, chloroform, isopropyl alcohol, 1xPBS, Reverse transcription kit, Fluorescent dye and other reagents