RNA Binding Protein Immunoprecipitation (RIP) Kit

• Cat.No.: KITMI-RP09
• Product Overview: RIP technique (RNA Binding Protein Immunoprecipitation) is used to investigate the binding of RNA and protein in the cell. This technology mainly uses specific antibodies of the target protein to precipitate the corresponding RNA-protein complex, and then the RNA is separated and purified for q-PCR verification or high-throughput sequencing analysis.

Specification

• Size: 6 tests
• Storage: Antibodies and protease inhibitors need to be stored at -20°C and the rest stored at 4°C
• Applications: Study the protein and RNA interactions
• Notes: 6 tests refer to 6 times of single group (1 group of IP or 1 group of IgG immunoprecipitation experiment. The following procedure includes 1 group of IgG and 1 group of IP, which consumes 2 test reagents
• Shipping: On Ice
• Kit Components: RIP Lysis Buffer: 3.6mL
  Protease Inhibitor (100X): 15uL
  RNase Inhibitor: 35uL
  Protein A/G Magnetic Beads: 200uL
  Normal Rabbit IgG (1mg/mL): 30uL
  Normal Mouse IgG (1mg/mL): 30uL
  RIP Wash Buffer: 50mL
  0.5 M EDTA: 400uL
  Salt Solution: 600uL
  DEPC H2O: 400uL
  Elution Buffer : 800uL
• Materials Required but Not Supplied: Trizol, 75% ethanol, chloroform, isopropyl alcohol, Reverse transcription kit and fluorescent dye