RFP Magnetic CoIP Agarose

• Cat.No.: RFP-02A
• Product Overview: Immunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to magnetic agarose beads..
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Ready-to-use
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Binding capacity: 22.5 μg of recombinant RFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 μm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 5 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!