Recombinant Yellow Fever Virus Envelope Protein, sFc-tagged

• Cat.No.: YEL-625
• Product Overview: This Yellow Fever virus Envelope protein has been manufactured in insect cells in response to the need for a highly purified, concentrated protein for use in further vaccine development and serological based diagnostic assays.

Specification

• Tag: sheep Fc
• Background: Translation of the positive-stranded viral genomic RNA in infected cells results in the synthesis of a polyprotein precursor of 3411 amino acids, which when proteolytically processed, generates 10 viral proteins. These are (from the amino- to carboxi-terminus of the precursor polyprotein): C; prM/M; E; NS1; NS2A; NS2B; NS3; NS4A; NS4B and NS5. The first three are viral structural proteins that form the virus particle together with the RNA molecule, namely capsid (C, 12–14 kDa), membrane (M of 8 kDa, its precursor prM of 18–22 kDa), and envelope (E, 52–54 kDa). These are encoded in the first quarter of the genome. The remainder of the genome encodes the non-structural proteins (NS), numbered from 1 to 5 (NS1 to NS5) in accordance with the order of synthesis.
  E protein is required for receptor binding and viral attachment, fusion, penetration, hemagglutination, host range and cell tropism. It also has an important role in immunological anti-virus response, eliciting neutralizing antibodies and inducing protective response. Native E protein forms homodimers and it undergoes conformational rearrangement into a fusogenic homotrimer after entering cells by receptor-mediated endocytosis. This conformational change occurs in the lower pH environment of the endosome where viral lipid envelope fusion with endosomal membrane, releases the nucleocapsids into the cells cytoplasm. Each E protein monomer has a molecular mass of 50-55 kDa and has three distinct domains: domain I, II and III. Domain III is the immunoglobulin-like receptor binding domain and is recognized by virus-neutralizing antibodies, making it a target for diagnostic assays.
  Yellow fever continues to be a public health concern inmany countries of Africa and the Americas. It is estimated that 200 000 cases and 30 000 deaths are attributable to yellow fever annually.
• Formulation: Presented in Dulbecco's phosphate buffered saline (DPBS) pH 7.4.
• Notes: This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
• Type: Recombinant
• ClassID 1: Infectious Disease

Gene Information

• Synonyms: Yellow Fever Virus Envelope