Recombinant Sudan Ebola Virus Glycoprotein, aa 33-632, C-His tagged

• Cat.No.: GP-13S
• Product Overview: 6×His tagged glycoprotein (GP) (amino acid 33-632) of Sudan ebolavirus (Nakisamata) expressed in 293 cells.
  Glycosylated recombinant viral protein expressed and purified from 293 cells

Specification

• Species: Sudan Ebola Virus
• Source: HEK293
• Tag: His
• Protein Length: 33-632
• Description: The Ebola virus (EBOV) is a mononegavirus which contains a 19 kb single-stand RNA encoding seven proteins. Rates of genetic change of ebolavirus are 100 times slower than influenza A in humans, but on the same magnitude as those of hepatitis B. The main Ebolavirus glycoprotein (GP) is the only viral protein found on the surface of the Ebola virion and is therefore responsible for mediating attachment and entry of the virus into host cells. The produced GP protein (~120 kDa) is derived from the sequence of a recent Sudan Ebolavirus from Uganda in 2011.
• AA Sequence: MPLGVVTNSTLEVTEIDQLVCKDHLASTDQLKSVGLNLEGSGVSTDIPSATKRWGFRSGVPPKVVSYEAGEWAENCYNLEIKKPDGSECLPPPPDGVRGFPRCRYVHKAQGTGPCPGDYAFHKDGAFFLYDRLASTVIYRGVNFAEGVIAFLILAKPKETFLQSPPIREAVNYTENTSSYYATSYLEYEIENFGAQHSTTLFKIDNNTFVRLDRPHTPQFLFQLNDTIHLHQQLSNTTGRLIWTLDANINADIGEWAFWENKKNLSEQLRGEELSFEALSLNETEDDDAASSRITKGRISDRATRKYSDLVPKNSPGMVPLHIPEGETTLPSQNSTEGRRVGVNTQETITETAATIIGTNGNHMQISTIGMRPSSSQIPSSSPTTAPSPEAQTPTTHTSGPSVMATEEPTTPPGSSPGPTTEAPTLTTPENITTAVKTVLPQESTSNGLITSTVTGILGSLGLRKRSRRQTNTKATGKCNPNLHYWTAQEQHNAAGIAWIPYFGPGAEGIYTEGLMHNQNALVCGLRQLANETTQALQLFLRATTELRTYTILNRKAIDFLLRRWGGTCRILGPDCCIEPHDWTKNITDKINQIIHDFIDHHHHHH
• Endotoxin: < 0.01 EU/μg of purified protein by LAL test
• Purity: > 96% (by SDS-PAGE)
• Applications: Western Blot, antigen, ELISA, etc.
• Storage: Store at -20 centigrade; Stable for 1-months from the date of shipment when kept at 4 oC. Nonhazardous, no MSDS required.
• Concentration: 1 μg/μL in PBS (20% glycerol, 0.1% sodium azide)
• References: 1. Shoemaker, T, et al. Reemerging Sudan ebola virus disease in Uganda, 2011. Emerging Infect. Dis., 18: 1480-1483, 2012

Gene Information

• Official Symbol: GP
• Synonyms: GP; Glycoprotein