Recombinant Streptococcus pyogenes Cas9-GFP Protein

• Cat.No.: Cas9-16S
• Product Overview: Recombinant Streptococcus pyogenes Cas9-GFP Protein, fused with enhanced GFP was expressed in E. coli, 3X NLS.
  Highly specific
  Highly active
  Ready-to-inject/transfect

Specification

• Description: CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
• Source: E. coli
• Species: Streptococcus pyogenes
• Form: Lyophilized powder
• Molecular Mass: ~194 kDa
• Purity: ≥ 90% (SDS-PAGE)
• Applications: Functional Genomics
  Target Validation
  Genome Editing
  Fluorescence Microscopy
  Flow Cytometry
• Storage: At −20 centigrade.
• Reconstitution: Lyophilized S. pyogenes Cas9-GFP protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
• Shipping: Wet ice

Gene Information

• Official Symbol: Cas9
• Synonyms: Cas9-EGFP, SpCas9-EGFP, SpCas9-GFP