Recombinant Staphylococcus aureus walK Protein (Asn35-Gln182), N-His tagged

• Cat.No.: walK-5760S
• Product Overview: Recombinant Staphylococcus aureus walK (Asn35-Gln182) fused with the N-terminal His tag was expressed in E. coli.

Specification

• Species: Staphylococcus aureus
• Source: E.coli
• Tag: His
• ProteinLength: Asn35-Gln182
• Description: Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 18.95 kDa
• Purity: > 90 % as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in 50 mM Tirs, pH 8.0, 150 mM Nacl or lyophilized from 50 mM Tirs, pH 8.0, 150 mM Nacl.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Official Symbol: walK
• Synonyms: Sensor protein kinase WalK; walK; EC:2.7.13.3; vicK; MW0019
• UniProt ID: Q7A215