Recombinant SARS Spike RBD protein, His-Avi-tagged

• Cat.No.: Spike-2433S
• Product Overview: Recombinant SARS Spike RBD protein(P59594)(Arg306-Phe527), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: SARS
• Source: HEK293
• Tag: Avi&His
• Protein Length: Arg306-Phe527
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.