Recombinant SARS-CoV-2 Spike RBD (K417N, E484K, N501Y) Protein, Fc-tagged

• Cat.No.: S-443S
• Product Overview: Recombinant SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation K417N, E484K, N501Y) (Arg319-Phe541) was expressed in HEK293 cell with the Fc region of human IgG1 at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.351) which emerged in the South Africa.

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Tag: Fc
• ProteinLength: 319-541
• Predicted N Terminal: Ser
• Molecular Mass: The recombinant SARS-CoV-2 Spike RBD consists of 462 amino acids and predicts a molecular mass of 51.94 kDa. As a result of glycosylation, it migrates as an approximately 56.01 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
  Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4.
  Please contact us for any concerns or special requirements.
  Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• mRNA Refseq: MN908947
• Protein Refseq: YP_009724390