Recombinant SARS-CoV-2 Spike Protein(RBD), His-tag, GMP Grade

• Cat.No.: Spike-038THP
• Product Overview: Recombinant SARS-CoV-2 Spike Protein(RBD) (Arg319-phe541) was fused with His tag, produced in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Arg319-phe541
• Description: The single-positive-strand RNA genome of SARS-CoV-2, encodes four major structural proteins: spike (S), envelope (E), membrane (M) and nucleocapsid (N). The spike protein comprises an N-terminal (S1) subunit, which contains the roughly 200-residue receptor binding domain (RBD), and a C-terminal subunit (S2), which contains the fusion protein. The RBD of SARS-CoV-2 binds more tightly to the extracellular domain of angiotensin-converting enzyme 2 (ACE2) than the homologous SARS-CoV (2002) RBD. The higher affinity results from sequence changes in RBD and this has been proposed to underlie the higher transmissibility of SARS-CoV-2. The previously determined structures showed that SARS-CoV RBD contains a core structure and a receptor-binding motif (RBM) and that the RBM binds to the outer surface of the claw-like structure of ACE2. Importantly, two virus-binding hot spots on human ACE2 has been identified. A number of naturally selected RBM mutations occurred near these two virus-binding hot spots, and these residues largely determined the host range of SARS-CoV.
• Form: Phosphate Buffered Saline, 5%mannital, 0.05% Tween20, pH7.4
• Molecular Mass: 26.20 kDa
• Endotoxin: >95% pure (SDS-PAGE).
• Storage: For long term storage, the product should be stored at -20°C or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2