Recombinant SARS-CoV-2 (L18F, D80A, D215G, del242-244, K417N, E484K, N501Y, D614G, A701V Mutant) Stabilized Spike Glycoprotein (Trimeric), His-Strep-tagged

• Cat.No.: S-307S
• Product Overview: SARS-CoV-2 (B.1.351) stabilized spike, a full-length spike glycoprotein ("South African variant"), relative to Wuhan-Hu-1 contains following amino acid changes: L18F, D80A, D215G, del242-244, K417N, E484K, N501Y, D614G, A701V with a His-2xStrep-tag at C-terminus was expressed in HEK293 and purified by affinity chromatography.
  The protein contains six proline amino acid changes (commonly known as Hexapro) to stabilize it in a trimeric pre-fusion state. The furin cleavage site has been altered to ‘SRAS' and deactivated.

Specification

• Species: SARS-COV-2
• Source: HEK293
• Tag: His&Strep
• Protein Length: 1-1205
• Description: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). The sequence WIV04/2019, belonging to the GISAID S clade / PANGOLIN A lineage / Nextstrain 19B clade, is believed to be the original sequence infecting humans. However, there are many thousands of variants of SARS-CoV-2 and subtypes of the virus can be placed into much larger groupings such as lineages or clades.
  The 501.V2 variant, also known as 501.V2, 20H/501Y.V2 (formerly 20C/501Y.V2), or lineage B.1.351, was first detected in South Africa in December 2020. It was reported that the prevalence of the variant was higher among young people with no underlying health conditions, more frequently resulting in serious illness compared to other variants. It was speculated that this variant may be driving a second wave of the COVID-19 epidemic in the country due to it spreading at a more rapid pace than other earlier variants of the virus. Routine sequencing by South African health authorities found that this new SARS-CoV-2 variant had largely replaced other SARS-CoV-2 viruses circulating in the Eastern Cape, Western Cape, and KwaZulu-Natal provinces. The 501.V2 variant contains several mutations that allow it to attach more easily to human cells, namely N501Y, K417N, and E484K and preliminary studies suggest the variant is associated with a higher viral load, which may suggest potential for increased transmissibility. The N501Y mutation is also present in the UK Lineage B.1.1.7. This mutation is of concern because it is located in the SARS-CoV-2 (501.V2) spike RBD, the viruses receptor-binding domain and increases binding to the receptor ACE2 (angiotensin converting enzyme 2).
• Form: Liquid
• Molecular Mass: Expected Molecular Weight: 140 kDa
  Observed Molecular Weight: 180 kDa
• Purity: Greater than 95% purity.
• Storage: Short Term Storage: -80 centigrade
  Long Term Storage: -80 centigrade
  Can be frozen
• Storage Buffer: DPBS
• Shipping: Dry Ice

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• mRNA Refseq: MN908947
• Protein Refseq: YP_009724390