Recombinant SARS-CoV-2 (B.1.617.2; AY.1, AY.2, AY.3) Spike Protein, His/Strep-tagged

• Cat.No.: S-998S
• Product Overview: Recombinant SARS-CoV-2 (B.1.617.2; AY.1, AY.2, AY.3) Spike Protein with a His/Strep tag was expressed in HEK293.
  SARS-CoV-2 Spike protein containing following amino acid changes: T19R, T95I, G142D, E156G, del157-158, W258L, N417K, L452R, T478K, D614G, P681R, D950N. The protein is stabilized as a pre-fusion trimer due to six amino acid changes in the S2 portion of Spike (HexaPro). The furin cleavage site has been mutated to GSAS. His-2xStrep-tag, C-terminus

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Tag: His&Strep
• Protein Length: 16-1205
• Description: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
• Form: Liquid
• Molecular Mass: Expected Molecular Weight: 140 kDa
  Observed Molecular Weight: 180 kDa
• Purity: > 95 %
• Storage: Short Term Storage: -80 centigrade
  Long Term Storage: -80 centigrade
  Can be frozen, but avoid multiple freeze-thaw cycles.
• Concentration: 0.70 mg/mL
• Storage Buffer: DPBS

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• Protein Refseq: YP_009724390