Recombinant SARS Coronavirus Nucleoprotein, His-tagged

• Cat.No.: N-130V
• Product Overview: Recombinant SARS Coronavirus Nucleoprotein (2-422aa), fused to His-tag at N-terminus, was expressed in mammalian HEK293 cells and purified by immobilised metal affinity and ion exchange chromatography.

Specification

• Species: SARS-CoV
• Source: HEK293
• Protein Length: 421
• Description: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• Form: Liquid. 50mM HEPES, pH8.0, 280mM NaCl.
• Applications: Vaccine research and immunoassay development.
• Storage: Store at -80 centigrade, Avoid freeze/thaw cycle.
• Concentration: 1.30 mg/ml
• Shipping: Dry Ice.

Gene Information

• Synonyms: GU280_gp10