Recombinant Saccharomyces cerevisiae GRE3 Protein (M1-A327), His tagged

• Cat.No.: GRE3-0089H
• Product Overview: Recombinant Saccharomyces cerevisiae 6His-TEV-GG-NADPH-dependent aldose reductase(M1-A327 end) Protein was expressed in E. coli.

Specification

• Species: S.cerevisiae
• Source: E.coli
• Tag: His
• ProteinLength: M1-A327
• Description: Aldose reductase with a broad substrate specificity. Reduces the cytotoxic compound methylglyoxal (MG) to acetol and (R)-lactaldehyde under stress conditions. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. In pentose-fermenting yeasts, aldose reductase catalyzes the reduction of xylose into xylitol. The purified enzyme catalyzes this reaction, but the inability of S.cerevisiae to grow on xylose as sole carbon source indicates that the physiological function is more likely methylglyoxal reduction (Probable).
• Form: Liquid
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: GRE3 trifunctional aldehyde reductase/xylose reductase/glucose 1-dehydrogenase (NADP(+)) [ Saccharomyces cerevisiae S288C ]
• Official Symbol: GRE3
• Synonyms: GRE3; trifunctional aldehyde reductase/xylose reductase/glucose 1-dehydrogenase (NADP(+)); trifunctional aldehyde reductase/xylose reductase/glucose 1-dehydrogenase (NADP(+)); EC 1.1.1.21; Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by HOG pathway; overexpression allows xylose fermentation in strains expressing heterologous xylitol dehydrogenase and xylulokinase; protein abundance increases in response to DNA replication stress
• Gene ID: 856504
• mRNA Refseq: NM_001179234
• Protein Refseq: NP_011972
• UniProt ID: P38715