Recombinant Rhesus PCSK9 Protein (Met1-Gln692), MIgG1 Fc-tagged

• Cat.No.: PCSK9-486R
• Product Overview: Recombinant Rhesus PCSK9 Protein (NP_001106130.1) (Met1-Gln692) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of mouse IgG1 at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: mFc
• Protein Length: Met1-Gln692
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
• Predicted N Terminal: Gln 31
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rhesus PCSK9 consists of 896 amino acids and predicts a molecular mass of 97.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: PCSK9
• Synonyms: PC9
• Gene ID: 717147
• mRNA Refseq: NM_001112660
• Protein Refseq: NP_001106130
• MIM: 607786
• UniProt ID: A8T666