Recombinant Rhesus macaque CFD protein, His-tagged

• Cat.No.: CFD-7854R
• Product Overview: Recombinant Rhesus macaque CFD protein(H9EXC1-1)(Gln21-Ala253), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rhesus macaque
• Tag: C-His
• Protein length: Gln21-Ala253
• Form: Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque Complement Factor D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.