Recombinant Rat Sirpa protein, His-tagged

• Cat.No.: Sirpa-195R
• Product Overview: Recombinant rat SIRPA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: His
• Description: SIRPA, also known as tyrosine-protein phosphatase non-receptor type substrate 1, is an immunoglobulin-like cell surface receptor for CD47. It supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. It is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 38.5kDa (350aa)57-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: KELKVTQADK SVSVAAGDSA TLNCTVSSLT PVGPIKWFKG EGQNRSPIYS FIGGEHFPRI TNVSDATKRN NMDFSICISN VTPEDAGTYYCVKFQKGIVE PDTEIKSGGG TTLYVLAKPS SPEVSGPDSR GSPGQTVNFT CKSYGFSPRN ITLKWLKNGK ELSHLETTIS SKSNVSYNISSTVSVKLSPE DIHSRVICEV AHVTLEGRPL NGTANFSNII RVSPTLKITQ QPLTPASQVN LTCQVQKFYP KALQLNWLEN GNLSRTDKPEHFTDNRDGTY NYTSLFLVNS SAHREDVVFT CQVEHDSQPA ITENHTVRAF AHSSSGGSME TIPDNNAYYN WNVEHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Sirpa signal-regulatory protein alpha [ Rattus norvegicus ]
• Official Symbol: Sirpa
• Synonyms: SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; sirp-alpha-1; SHP substrate 1; inhibitory receptor SHPS-1; macrophage fusion receptor; signal-regulatory protein alpha-1; CD172 antigen-like family member A; macrophage membrane protein MFP150; brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); Bit; Ptpns1; SHPS-1
• Gene ID: 25528
• mRNA Refseq: NM_013016
• Protein Refseq: NP_037148
• Pathway: Cell-Cell communication, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem
• Function: SH3 domain binding; protein phosphorylated amino acid binding; protein phosphorylated amino acid binding