Recombinant Rat Nphs1 protein, His & GST-tagged

• Cat.No.: Nphs1-5642R
• Product Overview: Recombinant Rat Nphs1 aa. (Pro39~Gly106 (Accession # Q9R044)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease.
• Source: E. coli
• Species: Rat
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro39~Gly106
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Nphs1 NPHS1 nephrin [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Nphs1
• Synonyms: Nphs1; NPHS1 nephrin; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); renal glomerulus-specific cell adhesion receptor
• Gene ID: 64563
• mRNA Refseq: NM_022628.1
• Protein Refseq: NP_072150.1
• UniProt ID: Q9R044