Recombinant Rat Lag3 Protein, His-tagged, FITC conjugated

• Cat.No.: Lag3-39RF
• Product Overview: FITC conjugated recombinant Rat Lag3 (Q5BK54) (Met1-Leu442), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant rat LAG3 comprises 430 amino acids and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: 430
• N-terminal Sequence Analysis: Gly 24
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with FITC via amines
  Excitation source: 488 nm spectral line, argon-ion laser
  Excitation Wavelength: 488 nm
  Emission Wavelength: 535 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM NaAc, 500 mM NaCl, 10% Glycerol, 0.02% tween 20, pH 5.5. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Lag3 lymphocyte-activation gene 3 [ Rattus norvegicus ]
• Official Symbol: Lag3
• Gene ID: 297596
• mRNA Refseq: NM_212513
• Protein Refseq: NP_997678