Recombinant Rat Interleukin 12b

• Cat.No.: Il12b-86R
• Product Overview: Recombinant Rat Interleukin-12 produced inSf9insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The IL-12 is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: Non
• Description: IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.8% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein (1 mg/ml) was lyophilized with no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀was determined by the stimulation of IFN-gamma production by murine splenocyes co-stimulated with IL-12 and was found to be < 0.1 ng/ml, corresponding to a specific activity of >1 x 10⁷.
• Stability: Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Il12b interleukin 12b [ Rattus norvegicus ]
• Synonyms: Il12b; interleukin 12b; Il12; interleukin-12 p40
• Gene ID: 64546
• mRNA Refseq: NM_022611
• Protein Refseq: NP_072133
• UniProt ID: Q62712
• Chromosome Location: 10q21
• Pathway: Allograft rejection; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; contributes_to cytokine activity; cytokine receptor activity; contributes_to growth factor activity; interleukin-12 alpha subunit binding; protein binding; protein homodimerization activity