Recombinant Rat GPT protein(Met1-Ser496), His-tagged

• Cat.No.: Gpt-294R
• Product Overview: Recombinant Rat Gpt1 (NP_112301.1) (Met 1-Ser 496) with a C-terminal polyhistidine tag was expressed in Insect Cells.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: His
• Protein Length: 1-496 a.a.
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 0.5mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat Gpt1 comprises 506 amino acids with a predicted molecular mass of 55 kDa. It migrates as an approximately 48 kDa band in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Liver enzyme delayed clearance in rat treated by CSF1 receptor specific antagonist Sotuletinib (2021)

Gene Information

• Gene Name: Gpt glutamic-pyruvate transaminase (alanine aminotransferase) [ Rattus norvegicus ]
• Official Symbol: GPT
• Synonyms: GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT 1; glutamic pyruvate transaminase; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; glutamic pyruvic transaminase (alanine aminotrasferase); Gpt1
• Gene ID: 81670
• mRNA Refseq: NM_031039
• Protein Refseq: NP_112301