Recombinant Rat GNAT3 Protein

• Cat.No.: GNAT3-720R
• Product Overview: Recombinant Rat GNAT3 protein (X65747) was co-expressed with G-protein β1γ2-subunits in Sf9 insect cells.

Specification

• Description: Gustducin is a transducin-like heterotrimeric guanine nucleotide-binding protein (G-protein) expressed in taste receptor cells (TRCs) of the tongue. It is also expressed in the stomach and intestine in brush cells that are scattered throughout the surface epithelium of the gut and share structural features of taste receptor cells of the tongue. Gustducin was demonstrated to be myristolated and was also palmitoylated in insect cells. Biochemical and genetic studies have implicated gustducin-α as a key component in the transduction of both bitter and sweet taste. Gustducin mediates two responses, i.e. a decrease in cyclic nucleotide monophosphates via activation of phosphodiesterase 1A by gustducin-α and activation of phospholipase C-β by released Gβγ subunits. Gustducin-α has close structural similarity with the visual G-protein, transducin-α. Gustducin-α reconstituted with the G-protein subunits Gβ1γ2 was prepared from Sf9 cells coinfected with gustducin-α and Gβ1γ2-encoding baculoviruses.
• Source: Insect cell
• Species: Rat
• Form: 75 mM Tris-HCl pH 7.4, 12.5 mM MgCl2 and 1 mM EDTA
• Molecular Mass: 40 + 36 kDa
• Storage: Store at -80 centigrade, avoid freeze/thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Gnat3 guanine nucleotide binding protein, alpha transducing 3 [ Rattus norvegicus ]
• Official Symbol: GNAT3
• Synonyms: GDCA
• Gene ID: 346562
• mRNA Refseq: NM_001102386.1
• Protein Refseq: NP_001095856.1
• MIM: 139395
• UniProt ID: A8MTJ3