Recombinant Rat FAS protein(Met1-Lys170), hFc-tagged

• Cat.No.: FAS-547R
• Product Overview: Recombinant Rat FAS (NP_631933.2) (Met1-Lys170) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: 1-170 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human His-FASLG at 10 μg/ml (100 μl/well) can bind rat FAS-Fc. The EC50 of rat FAS-Fc is 0.22-0.52 μg/ml.
• Molecular Mass: The recombinant rat FAS/Fc is a disulfide-linked homodimer. The reduced monomer comprises 390 amino acids and has a predicted molecular mass of 43.7 kDa. The apparent molecular mass of the protein is approximately 57 and 36 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fas Fas (TNF receptor superfamily, member 6) [ Rattus norvegicus ]
• Official Symbol: FAS
• Synonyms: FAS; Fas (TNF receptor superfamily, member 6); tumor necrosis factor receptor superfamily member 6; CD95 antigen; Fas receptor; apo-1 antigen; FASLG receptor; Fas antigen (ATP1); apoptosis-mediating surface antigen FAS; Tumor necrosis factor receptor superfamily, member 6; Tnfrsf6
• Gene ID: 246097
• mRNA Refseq: NM_139194
• Protein Refseq: NP_631933