Recombinant Rat Erbb2 Protein (Ala67-Val323), C-His tagged

• Cat.No.: Erbb2-6839R
• Product Overview: Recombinant rat Erbb2 (Ala67-Val323) fused with the C-terminal His tag was expressed in mammalian cells.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ala67-Val323
• Description: Enables Hsp90 protein binding activity; protein tyrosine kinase activity; and ubiquitin protein ligase binding activity. Involved in several processes, including animal organ development; nervous system development; and positive regulation of intracellular signal transduction. Located in several cellular components, including basolateral plasma membrane; lateral loop; and microvillus. Biomarker of congestive heart failure and median neuropathy. Human ortholog (s) of this gene implicated in carcinoma (multiple); dilated cardiomyopathy; high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Orthologous to human ERBB2 (erb-b2 receptor tyrosine kinase 2).
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 127.27 kDa
• Purity: > 90 % as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS or lyophilized from PBS.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: Erbb2 erb-b2 receptor tyrosine kinase 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Erbb2
• Synonyms: Erbb2; erb-b2 receptor tyrosine kinase 2; neu; HER2/neu; receptor tyrosine-protein kinase erbB-2; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); C-erbB-2; NEU proto-oncogene; epidermal growth factor receptor-related protein; p185erbB2; p185neu; proto-oncogene NEU; proto-oncogene c-ErbB-2; receptor tyrosine-protein kinase erbB-2-like; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
• Gene ID: 24337
• mRNA Refseq: NM_017003
• Protein Refseq: NP_058699
• UniProt ID: P06494

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.