Recombinant Rat Epcam protein(Met1-Thr266), His-tagged

• Cat.No.: Epcam-7482R
• Product Overview: Recombinant Rat EPCAM (O55159) (Met1-Thr266) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Protein length: 1-266 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat EPCAM comprises 254 amino acids and predicts a molecular mass of 29.2 kDa. The apparent molecular mass of the recombinant protein is approximately 39 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epcam epithelial cell adhesion molecule [ Rattus norvegicus ]
• Official Symbol: Epcam
• Synonyms: EPCAM; epithelial cell adhesion molecule; ep-CAM; epithelial glycoprotein 314; tumor-associated calcium signal transducer 1; Egp314; Tacstd1
• Gene ID: 171577
• mRNA Refseq: NM_138541
• Protein Refseq: NP_612550