Recombinant Rat Comt Protein, His-tagged

• Cat.No.: Comt-829R
• Product Overview: Recombinant Rat Comt Protein (Arg20-Ser264) with His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Arg20-Ser264
• Description: Enables catechol O-methyltransferase activity. Involved in several processes, including learning or memory; negative regulation of smooth muscle cell proliferation; and regulation of cellular amine metabolic process. Located in several cellular components, including cell body; dendritic spine; and postsynaptic membrane. Used to study Parkinsonism and hypertension. Biomarker of hypertension. Human ortholog(s) of this gene implicated in several diseases, including attention deficit hyperactivity disorder; autoimmune disease of skin and connective tissue (multiple); bulimia nervosa; cognitive disorder (multiple); and reproductive organ cancer (multiple). Orthologous to human COMT (catechol-O-methyltransferase).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.7 kDa; Accurate Molecular Mass: 29 kDa
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Comt catechol-O-methyltransferase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Comt
• Synonyms: COMT; catechol-O-methyltransferase; catechol O-methyltransferase; catecholamine-O-methyltransferase
• Gene ID: 24267
• mRNA Refseq: NM_012531
• Protein Refseq: NP_036663
• UniProt ID: P22734