Recombinant Rat Comt protein, His-tagged

• Cat.No.: Comt-1671R
• Product Overview: Recombinant Rat Comt aa. (Gly63~Ser264) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Gly63~Ser264
• Description: Catalyzes the conversion of S-adenosyl-L-methionine and catechol to S-adenosyl-L-homocysteine and guaiacol.
• Form: Freeze-dried powder
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.4

Gene Information

• Gene Name: Comt catechol-O-methyltransferase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Comt
• Synonyms: Comt; catechol-O-methyltransferase; catechol O-methyltransferase; catecholamine-O-methyltransferase
• Gene ID: 24267
• mRNA Refseq: NM_012531.2
• Protein Refseq: NP_036663.1
• UniProt ID: P22734