Recombinant Rat Apc Protein, His-tagged

• Cat.No.: Apc-202R
• Product Overview: Recombinant Rat Apc Protein (Ile213-Lue461) with N-His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Ile213-Lue461
• Description: Enables microtubule binding activity. Involved in several processes, including cellular response to nerve growth factor stimulus; negative regulation of cardiac muscle cell proliferation; and regulation of glutamate metabolic process. Located in several cellular components, including axonal growth cone; cell body fiber; and lateral plasma membrane. Part of protein-containing complex. Colocalizes with neuron projection and synapse. Used to study anemia; colon adenocarcinoma; colon cancer; and familial adenomatous polyposis. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); endometrial cancer (multiple); familial adenomatous polyposis 1; hereditary desmoid disease; and medulloblastoma. Orthologous to human APC (APC regulator of WNT signaling pathway).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.3 kDa
  Accurate Molecular Mass: 28&56 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apc adenomatous polyposis coli [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Apc
• Synonyms: APC; adenomatous polyposis coli; adenomatous polyposis coli protein; adenomatosis polyposis coli; RATAPC
• Gene ID: 24205
• mRNA Refseq: NM_012499
• Protein Refseq: NP_036631
• UniProt ID: P31394