Recombinant Rat Alox5 Protein, His-tagged

• Cat.No.: Alox5-121R
• Product Overview: Recombinant Rat Alox5 Protein (Leu373-Leu535) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable arachidonate 5-lipoxygenase activity and iron ion binding activity. Involved in several processes, including positive regulation of cytochrome-c oxidase activity; positive regulation of vasoconstriction; and response to hyperoxia. Located in several cellular components, including dendrite; nuclear envelope; and sarcolemma. Used to study several diseases, including brain ischemia; hypertension (multiple); pleurisy; portal hypertension; and pulmonary edema. Biomarker of pulmonary hypertension. Human ortholog(s) of this gene implicated in artery disease (multiple); asthma (multiple); pancreatic cancer; and pulmonary tuberculosis. Orthologous to human ALOX5 (arachidonate 5-lipoxygenase).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20 kDa
• Protein length: Leu373-Leu535
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Alox5 arachidonate 5-lipoxygenase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Alox5
• Synonyms: ALOX5; arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; 5 - Lipoxygenase; LOX5A
• Gene ID: 25290
• mRNA Refseq: NM_012822
• Protein Refseq: NP_036954
• UniProt ID: P12527