Recombinant Puumala Virus Glycoprotein 2 (Gc), hFc-tagged

• Cat.No.: PUU-543
• Product Overview: Recombinant Puumala virus glycoprotein 2 (Gc), corresponding to amino acids 660-1116 of the M segment polyprotein of PUUV, contains a C-terminal human IgG1 Fc-tag with a 15 AA glycine-serine linker. The fusion protein was co-expressed with the corresponding unloaded human IgG1 Fc chain.

Specification

• Tag: Fc
• Background: Puumala virus (PUUV) is an enveloped, single-stranded negative-sense RNA virus. PUUV is a Hantavirus that belongs to the Hantavirus genus, within the family Bunyaviridae. Hantaviruses are zoonotic pathogens that have global distribution. Rodents and small mammals act as natural reservoirs for Hantaviruses, with each Hantavirus strain being transmitted by a different rodent host species or related species.
  The hantavirus virion comprises three genome segments S, M and L, that encode the nucleocapsid (N) protein, glycoprotein precursor (GPC) and RNA-dependent RNA-polymerase, respectively (Schmaljohn, 2007). GPC is a polyprotein of 1133–1158 amino acid (aa) residues in length (Spiropoulou, 2011). Cotranslational cleavage of GPC (a 1133–1158 amino acid polyprotein) at a site C-terminal to a conserved 'WAASA' sequence by the cellular signal peptidase complex produces glycoproteins G1/Gn and G2/Gc, respectively (Löber, et al., 2001). These glycoproteins are responsible for virus attachment and entry into the host cells and are considered to be a major determinant of virus pathogenicity (Spiropoulou, 2011).
  The G1 and G2 cytoplasmic tails interact with virus encoded ribonucleoprotein (RNP) complexes and are involved in virion assembly (Schmaljohn, et al., 1985; Hepojoki, et al., 2012). They form a spike complex, which is located on the outer surface of the virion with each spike containing four molecules of both glycoproteins. Virus-membrane fusion activity has been associated with G2 through the identification of a fusion peptide which appears to be conserved across the Bunyaviridae (Battisti, et al., 2011). Chimeric VLPs have been described for the generation of virus-reactive mAbs against hantavirus G2 glycoprotein (Zvirbliene, et al., 2014) which will be a useful tool for the development of diagnostic assays.
• Purity: Greater than 90% purity by SDS-PAGE.
• Sequence Strain: Umea/hu
• Formulation: DPBS pH 7.4, sterile filtered
• Freezing: Can be frozen, but avoid multiple freeze/thaw cycles.
• Storage: Short Term Storage: +2 centigrade to +8 centigrade
  Long Term Storage: -80 centigrade
• Concentration: 0.19 mg/mL
• Notes: This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
• Type: Recombinant
• ClassID 1: Infectious Disease

Gene Information

• Synonyms: Puumala Virus Glycoprotein 2 (Gc)