Recombinant Porcine Interleukin 4

• Cat.No.: IL4-84P
• Product Overview: Recombinant Porcine IL-4 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615 Dalton. The IL-4 is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleikin-4 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED50 range= 1 to 4ng/ml. The biological activity is determined by measuring the dose dependent proliferation of human TF-1 cells. A concentration range of 0.1 to 10.0ng/ml is effective for most in vitro applications.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
• Stability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL4 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL4 interleukin 4 [ Sus scrofa ]
• Synonyms: interleukin 4; IL4
• Gene ID: 397225
• mRNA Refseq: NM_214123
• Protein Refseq: NP_999288
• UniProt ID: Q04745
• Chromosome Location: 2
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; T cell receptor signaling pathway