Recombinant Mouse Wdyhv1 Protein, Myc/DDK-tagged

• Cat.No.: Wdyhv1-6991M
• Product Overview: Purified recombinant protein of Mouse WDYHV motif containing 1 (Wdyhv1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 24.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wdyhv1 WDYHV motif containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Wdyhv1
• Synonyms: WDYHV1; WDYHV motif containing 1; protein N-terminal glutamine amidohydrolase; nt(Q)-amidase; N-terminal Gln amidase; WDYHV motif-containing protein 1; protein NH2-terminal glutamine deamidase; Ntaq1; AU014961; AW550036; 2410187C16Rik
• Gene ID: 76773
• mRNA Refseq: NM_029734
• Protein Refseq: NP_084010
• UniProt ID: Q80WB5