Recombinant Mouse Usp4 Protein, Myc/DDK-tagged

• Cat.No.: Usp4-6879M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.
• Molecular Mass: 126.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp4 ubiquitin specific peptidase 4 (proto-oncogene) [ Mus musculus (house mouse) ]
• Official Symbol: Usp4
• Synonyms: Usp4; ubiquitin specific peptidase 4 (proto-oncogene); U; Unp; mKIAA4155; F730026I20Rik; ubiquitin carboxyl-terminal hydrolase 4; deubiquitinating enzyme 4; ubiquitin specific protease 4 (proto-oncogene); ubiquitin thioesterase 4; ubiquitin thiolesterase 4; ubiquitin-specific-processing protease 4; ubiquitous nuclear protein; EC 3.4.19.12
• Gene ID: 22258
• mRNA Refseq: NM_011678
• Protein Refseq: NP_035808
• UniProt ID: P35123