Recombinant Mouse Uchl3 Protein, Myc/DDK-tagged

• Cat.No.: Uchl3-6820M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 26.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Uchl3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [ Mus musculus (house mouse) ]
• Official Symbol: Uchl3
• Synonyms: UCHL3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; ubiquitin thiolesterase; ubiquitin thioesterase L3
• Gene ID: 50933
• mRNA Refseq: NM_016723
• Protein Refseq: NP_057932
• UniProt ID: Q9JKB1