Recombinant Mouse Ube3c Protein, MYC/DDK-tagged

• Cat.No.: Ube3c-167M
• Product Overview: Purified recombinant protein of full-length mouse ubiquitin protein ligase E3C (Ube3c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 124 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube3c ubiquitin protein ligase E3C [ Mus musculus (house mouse) ]
• Official Symbol: Ube3c
• Synonyms: Ube3c; ubiquitin protein ligase E3C; AI853514; mKIAA0010; ubiquitin-protein ligase E3C; HECT-type ubiquitin transferase E3C; EC 2.3.2.26
• Gene ID: 100763
• mRNA Refseq: NM_133907
• Protein Refseq: NP_598668
• UniProt ID: Q80U95