Recombinant Mouse Ube2b Protein, Myc/DDK-tagged

• Cat.No.: Ube2b-6780M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2B (Ube2b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
• Molecular Mass: 17.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2b ubiquitin-conjugating enzyme E2B [ Mus musculus (house mouse) ]
• Official Symbol: Ube2b
• Synonyms: UBE2B; ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae); ubiquitin-conjugating enzyme E2 B; E214K; RAD6 homolog B; ubiquitin-protein ligase B; ubiquitin carrier protein B; HR6B; Rad6b; mHR6B; E2-14k; 2610301N02Rik
• Gene ID: 22210
• mRNA Refseq: NM_009458
• Protein Refseq: NP_033484
• UniProt ID: P63147