Recombinant Mouse Trmo Protein, Myc/DDK-tagged

• Cat.No.: Trmo-6666M
• Product Overview: Purified recombinant protein of mouse full-length tRNA methyltransferase O (Trmo), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m6t6A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 54.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trmo tRNA methyltransferase O [ Mus musculus (house mouse) ]
• Official Symbol: Trmo
• Synonyms: Trmo; tRNA methyltransferase O; Nap1; AV014846; 5830415F09Rik; tRNA (adenine(37)-N6)-methyltransferase; likely ortholog of H. sapiens chromosome 9 open reading frame 156 (C9orf156); nef-associated protein 1; thioesterase NAP1
• Gene ID: 74753
• mRNA Refseq: NM_029086
• Protein Refseq: NP_083362
• UniProt ID: Q562D6