Recombinant Mouse Trim11 Protein, Myc/DDK-tagged

• Cat.No.: Trim11-6643M
• Product Overview: Purified recombinant protein of mouse full-length tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.
• Molecular Mass: 85.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trim11 tripartite motif-containing 11 [ Mus musculus (house mouse) ]
• Official Symbol: Trim11
• Synonyms: Trim11; tripartite motif-containing 11; E3 ubiquitin-protein ligase TRIM11; RING-type E3 ubiquitin transferase TRIM11; tripartite motif-containing protein 11; EC 2.3.2.27
• Gene ID: 94091
• mRNA Refseq: NM_053168
• Protein Refseq: NP_444398
• UniProt ID: Q99PQ2