Recombinant Mouse TPP1 Protein (Met1-Pro562), His-tagged

• Cat.No.: TPP1-874M
• Product Overview: Recombinant Mouse TPP1 Protein (NP_034036.1) (Met1-Pro562) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Pro562
• Description: Tripeptidyl-peptidase 1 (TPP1 / CLN2) is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. TPP1 / CLN2 May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Defects in TPP1 / CLN2 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2), a form of neuronal ceroid lipofuscinosis which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.
• Predicted N Terminal: Thr 20
• Form: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.5, 10 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse TPP1 consists of 554 amino acids and predicts a molecular mass of 60.9 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Tpp1 tripeptidyl peptidase I [ Mus musculus ]
• Official Symbol: TPP1
• Synonyms: TPP1; tripeptidyl peptidase I; LPIC; TPP-1; Cln2; TPP-I
• Gene ID: 12751
• mRNA Refseq: NM_009906
• Protein Refseq: NP_034036
• UniProt ID: O89023