Recombinant Mouse Toll-like Receptor 4, Fc Chimera

• Cat.No.: Tlr4-2432M
• Product Overview: Recombinant N-terminal region of the extracellular domain of mouse TLR4 (aa 24-334) produced inHEK 293 cellsis fused at the C-terminus to the Fc portion of human IgG1 and a linker peptide (2 aa). 83kDa (SDS-PAGE).

Specification

• Species: Mouse
• Source: Human Cells
• Tag: Fc
• Protein Length: 24-334 a.a.
• Description: Toll-like receptor 4 is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. It detects lipopolysaccharide on Gram-negative bacteria and is thus important in the activation of the innate immune system. TLR4 has also been designated as CD284 (cluster of differentiation 284). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities.
• Source/Host: HEK 293 cells.
• Concentration: 1mg/ml after reconstitution.
• Purity: ≥90% (SDS-PAGE).
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
• Formulation: Lyophilized. Contains PBS.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
• Publications:
  Tannic acid prevents macrophage‐induced pro‐fibrotic response in lung epithelial cells via suppressing TLR4‐mediated macrophage polarization (2019)

Gene Information

• Gene Name: Tlr4 toll-like receptor 4 [ Mus musculus ]
• Synonyms: toll-like receptor 4; Lps; Ly87; Ran/M1; Rasl2-8; Tlr4; OTTMUSP00000000296; lipopolysaccharide response; toll-like receptor 4
• Gene ID: 21898
• mRNA Refseq: NM_021297
• Protein Refseq: NP_067272
• UniProt ID: Q9QUK6
• Chromosome Location: 4 C1; 4 33.0 cM
• Pathway: Chagas disease; Leishmaniasis; Toll-like receptor signaling pathway
• Function: lipopolysaccharide binding; phosphoinositide 3-kinase binding; protein binding; receptor activity; transmembrane receptor activity