Recombinant Mouse Tnr Protein, Myc/DDK-tagged

• Cat.No.: Tnr-6573M
• Product Overview: Purified recombinant protein of mouse full-length tenascin R (Tnr), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Theses interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediates inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 150 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tnr tenascin R [ Mus musculus (house mouse) ]
• Official Symbol: Tnr
• Synonyms: TNR; tenascin R; tenascin-R; neural recognition molecule J1-160/180; TN-R; janusin; restrictin; J1-tenascin
• Gene ID: 21960
• mRNA Refseq: NM_022312
• Protein Refseq: NP_071707
• UniProt ID: Q8BYI9