Recombinant Mouse Timp3 protein, His-tagged

• Cat.No.: Timp3-546M
• Product Overview: Recombinant Mouse Timp3 protein(Gly20~Pro211), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Gly20~Pro211
• Tag: N-His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.
• Bio-activity: Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. The activity of recombinant mouse TIMP3 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 °C for 1 hour and rmTIMP3 (MW: 23.25 KD) was diluted to different concentrations with the assay buffer. Mix 8 μl of rmTIMP3 curve dilutions, 12.8 μl of activated rhMMP-2, and 59.2 μl of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 °C. Loading 50 μl of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 μl of 20 μM substrate. Include a substrate blank containing 50 μl of assay buffer and 50 μl of 20 μM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The inhibition IC50 was <26 nM.
• Molecular Mass: 23.3kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >98%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Timp3 tissue inhibitor of metalloproteinase 3 [ Mus musculus ]
• Official Symbol: Timp3
• Synonyms: TIMP3; tissue inhibitor of metalloproteinase 3; metalloproteinase inhibitor 3; tissue inhibitor of metalloproteinases 3; Timp-3
• Gene ID: 21859
• mRNA Refseq: NM_011595
• Protein Refseq: NP_035725