Recombinant Mouse Tigit Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Tigit-2348MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Tigit (NP_001139797.1) extracellular domain (Met 1-Gly 141), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: 127
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse TIGIT comprises 127 amino acids and has a calculated molecular mass of 14.2 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus ]
• Official Symbol: Tigit
• Gene ID: 100043314
• mRNA Refseq: NM_001146325
• Protein Refseq: NP_001139797