Recombinant Mouse Thymus Cell Antigen 1, Theta, Fc Chimera

• Cat.No.: Thy1-2430M
• Product Overview: Recombinant extracellular domain of mouse Thy-1 (aa 20-131) produced inHEK 293 cellsis fused to the Fc portion of human IgG1. The integrin binding sequence RLD of mouse Thy-1 has been changed to RLE abolishing the binding of integrins, 48kDa (SDS-PAGE).

Specification

• Species: Mouse
• Source: Human Cells
• Tag: Fc
• Description: Thy-1 is a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in cells of the neuronal system, activited microvascular endothelial cells, fibroblasts and blood stem cells. Thy-1 is involved in cell adhesion. Thy-1 interacts with the integrin avB3 (CD51/CD61) and Mac-1 (CD11b/CD18). In astrocytes, binding of Thy-1 to β3-integrin promotes focal adhesion formation.
• Source/Host: HEK 293 cells.
• Concentration: 1mg/ml after reconstitution.
• Purity: ≥90% (SDS-PAGE).
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
• Formulation: Lyophilized. Contains PBS.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Gene Information

• Gene Name: Thy1 thymus cell antigen 1, theta [ Mus musculus ]
• Synonyms: thymus cell antigen 1, theta; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2; Thy1; Thy 1.2; ; theta; Thy 1.2; OTTMUSP00000018127
• Gene ID: 21838
• mRNA Refseq: NM_009382
• Protein Refseq: NP_033408
• UniProt ID: P01831
• Chromosome Location: 9 26.0 cM
• Function: Leukocyte transendothelial migration