Recombinant Mouse Thg1l Protein, Myc/DDK-tagged

• Cat.No.: Thg1l-6412M
• Product Overview: Purified recombinant protein of mouse full-length tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) (Thg1l), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Also functions as a guanyl-nucleotide exchange factor/GEF for the MFN1 and MFN2 mitofusins thereby regulating mitochondrial fusion. By regulating both mitochondrial dynamics and bioenergetic function, it contributes to cell survival following oxidative stress.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 35 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Thg1l tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) [ Mus musculus (house mouse) ]
• Official Symbol: Thg1l
• Synonyms: THG1L; tRNA-histidine guanylyltransferase 1-like (S. cerevisiae); probable tRNA(His) guanylyltransferase; nterphase cyctoplasmic foci protein 45; AA387658; RP23-298M7.6; 1700121M19Rik; 5730409G07Rik; MGC107321
• Gene ID: 66628
• mRNA Refseq: NM_001080969
• Protein Refseq: NP_001074438
• UniProt ID: Q9CY52