Recombinant Mouse Taok2 Protein, Myc/DDK-tagged

• Cat.No.: Taok2-6290M
• Product Overview: Purified recombinant protein of mouse full-length TAO kinase 2 (Taok2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. May affect microtubule organization and stability. May play a role in the osmotic stress-MAPK8 pathway. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14/p38 MAPK activation.
• Molecular Mass: 139.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Taok2 TAO kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Taok2
• Synonyms: TAOK2; TAO kinase 2; serine/threonine-protein kinase TAO2; TAO kinase 2TAO kinase 2; thousand and one amino acid protein 2; PSK; PSK1; TAO1; TAO2; MAP3K17; mKIAA0881; B230344N16; 1110033K02Rik; KIAA0881
• Gene ID: 381921
• mRNA Refseq: NM_001163775
• Protein Refseq: NP_001157247
• UniProt ID: Q6ZQ29