Recombinant Mouse Spout1 Protein, Myc/DDK-tagged

• Cat.No.: Spout1-2439M
• Product Overview: Purified recombinant protein of mouse full-length DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level.
• Molecular Mass: 20.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Spout1 SPOUT domain containing methyltransferase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Spout1
• Synonyms: Spout1; SPOUT domain containing methyltransferase 1; CENP-32; D2Wsu81; AL033358; D2Wsu81e; 9930028P05; putative methyltransferase C9orf114 homolog; centromere protein 32; kinetochore-associated protein
• Gene ID: 227695
• mRNA Refseq: NM_172660
• Protein Refseq: NP_766248
• UniProt ID: Q3UHX9